She along with Trixie Tang was fused together into Trixootie after Timmy made a rule-free wish with his Magic Muffin. Trixootie gave Vicky a swirlie as payback for ruining Tootie's birthdays, and also intimidated Mr. Crocker and Francis.
Tootie along with Timmy, his fairies, Chester and A.J. introduce Ivan to Mark Chang and later on helped defeat Princess Mandie and prevent her from destroying the Earth after Ivan was exposed while he was disguised as Mark.
Tootie supports Ivan and Timmy after being punished for using "sentence enhancers" in school. And after hearing Vicky saying all thirteen bad words, she and the boys rushed to her house and tell her mother. It was revealed earlier in the episode that Timmy does not attend David Mulligan's class because of Tootie.
Tootie was disgusted and shocked seeing Timmy in a romantic relationship with her sister, and had a nightmare where her boyfriend married Vicky. She and Ivan eventually decide to spy on Timmy and Vicky. When Vicky kidnaps Timmy, Tootie dons her Ninja of Love costume and turns off the lights in Vicky's room, beating her up in the dark and tying her up so that Ivan could use Cupid's Anti-Love plungers to make Vicky fall out of love with Timmy. Timmy was amazed at what Tootie did to her sister and after kissing her, he said to Tootie that he'll always prefer her over Vicky as his girlfriend.
Tootie, Timantha and the rest of the Cream Puffs challenge the Squirrely Scouts into finding the rabid red-headed squirrel. Little did they know, it was Neptunia who was surrounded by her phobia of standing in the middle of the forest and turned rabid.